Chapter 8 - Tests

Question 2

Test for PKU (6)

Answer 2

1. newborn screening2. Guthrie Test3. Automated Technique4. Ferric Chloride5. Phenistix6. HPLC

Question 3

PKU:most well known-positive: bacterial culture

Answer 3

Guthrie test

Question 4

PKU:positive for Ferric Chloride Test

Answer 4

blue-green

Question 5

PKU:Phenistix (+)

Answer 5

Blue-gray to green-gray

Question 6

PKU:reference method

Answer 6

HPLC

Question 7

Tyrosinuria Tests: (4)

Answer 7

1. Nitrosonaphthol Test2. Millon’s Test3. Ferric Chloride test4. Phenistix

Question 8

Tyrosinuria Tests:Nitrosonapthol color-specificity

Answer 8

orange-rednonspecific

Question 9

Tyrosinuria Tests:Millon’s TestColorreagent

Answer 9

red precipitateMercury in conc. HNO3

Question 10

Tyrosinuria Tests:Ferric Chloridecolor

Answer 10

transient green

Question 11

Tyrosinuria Tests:Phenistixcolor

Answer 11

transient green

Question 12

Melanuria tests:Ferric chloridecolor

Answer 12

gray or black precipitate

Question 13

Alkaptonuria test: (4)

Answer 13

1. Ferric chloride2. Benedict’s test3. Chromatography4. capillary electrophoresis

Question 14

Alkaptonuria tests:confirmatory tests (2)

Answer 14

chromotographycapillary electrophoresis

Question 15

Alkaptonuria tests:ferric chloridecolor

Answer 15

transient deep blue

Question 16

Alkaptonuria tests:benedict’s or clinitest

Answer 16

yellow precipitate

Question 17

Maple Syrup Urine Disease Test:(6)

Answer 17

1. Modified Guthrie test2. DNPH3. Amino acid chromatography4. Ferric chloride5. Nitroso-napthol6. Acetest

Question 18

Maple Syrup Urine Disease Test:DNPHcolor

Answer 18

yellow turbidity or precipitate

Question 19

Maple Syrup Urine Disease Test:confirmatory

Answer 19

amino acid chromatography

Question 20

Maple Syrup Urine Disease Test:ferric chloridecolor

Answer 20

green-gray

Question 21

Maple Syrup Urine Disease Test:nitro-napthol

Answer 21

red

Question 22

Maple Syrup Urine Disease Test:acetest

Answer 22

purple

Question 23

Methylamolnic Acidemia screening test and color

Answer 23

p-nitroaniline test - emerald green

Question 24

5-HydroxyIndoleacetic Acid Tests:(2)

Answer 24

1. Ferric Chloride2. Sjoerdsma Test

Question 25

5-HydroxyIndoleacetic Acid Tests:recommended specimen

Answer 25

first morning urine - prevent false-negative results

Question 26

5-HydroxyIndoleacetic Acid Tests:bananas, pineappples, tomatoesmedications: phenothiazines acetanilids.

Answer 26

Diet: food not allowed - rich in serotonin

Question 27

5-HydroxyIndoleacetic Acid Tests:Ferric chloridecolor

Answer 27

blue-green

Question 28

5-HydroxyIndoleacetic Acid Tests:Sjoerdmsa test-principle-color

Answer 28

1-nitroso-2-naphthol- purple to black

Question 29

Indicanuria tests: (3)

Answer 29

1. Obermayer's Test2. Jaffe's test3. Jolle's test

Question 30

Cystinuria:- more probable stone formers of the 2 modes of inheritance

Answer 30

4 amino acid reabsorption problem

Question 31

Cystinosis Test: (2)

Answer 31

1. cyanide-nitroprusside 2. Clinitest

Question 32

Cystinuria tests:cyanide-nitroprussidecolor

Answer 32

red-purple

Question 33

Cystinuria tests:clinitest

Answer 33

orange-red

Question 34

Homocystinuria test:(3)

Answer 34

1. newborn screening - mod. Guthrie2. Cyanide-nitropruside 3. silver nitroprusside

Question 35

Homocystinuria test:Cyanide-nitroprussidecolorspecificity

Answer 35

red-purplenonspecific

Question 36

Homocystinuria test:sliver nitroprussidecolorspecificity

Answer 36

red purple-follow-up test after positive cyanide-nitropruside=fresh urine should be used

Question 37

Porphyria Test: (2)

Answer 37

1. Ehrlich2. Fluorescence under UV 550-600 nm

Question 38

Porphyria Test:Fluorsecencecolor

Answer 38

violet, pink or red

Question 39

Mucopolysaccharide Disorder tests:acid-albumincolor

Answer 39

white precipitate

Question 40

Mucopolysaccharide Disorder tests:CTAB color

Answer 40

white precipitate

Question 41

Mucopolysaccharide Disorder tests:metachromatic staining spot test

Answer 41

blue dye cannot be washed

Question 42

Purine Disorder-Urine colortexture

Answer 42

Orange sand in diapers = uric acid

Question 43

Glactose and Fructose test:result- dipstick- clinitest

Answer 43

-dipstick = (-)-clinitest/copper reduction = (+)