Chromatography
is the scientific procedure used to separate the components of a compound so that they can be identified.
Chromatography inventor
M.S. Tswett
Science application of Chromatography
to analyze the components that make up different compounds (ie. proteins, pigments, foods)
Police application of Chromatography
to detect drugs in urine, alcohol in blood, explosives, poisons.
Liquid Chromatography
used to test water for pollution
Gas Chromatography
is used in airports to detect bombs
Thin-layer Chromatography
used to detect pesticides and insecticide residues in food
Paper Chromatography
used to separate dyes in ink samples and to separate pigments in plant leaves
Stationary phase in Paper Chromatography
The mixture to be separated is put on a piece of paper. The paper is called the stationary phase.
Mobile phase in Paper Chromatography
Water is passed over the stationary phase, going in one direction. The water is called the mobile phase.
Movement of components in Chromatography
The components in the mixture move with the mobile phase and are absorbed at different locations on the stationary phase.
Rf (Retention factor) formula
Rf (Retention factor) = D1 / D2 where D1 is the distance of the solute and D2 is the distance of the solvent
Color intensity in Chromatography
The lighter the color is = farther from the origin
