Chromogens used for HRP
DAB
AEC
Counterstain for DAB
Hematoxylin
Methyl green
Methyl blue
Counterstain for AEC
Hematoxylin
Methyl blue
Chromogen used for ALP
5-Bromo-4-Chloride-3-Indolyl-Phosphate (BCIP)
Fast Red TR Salt
Counterstain used for BCIP
Color product using BCIP
Bluish Violet
Black Violet
Color Product of Fast Red TR Salt
Rose color
Counterstain for Fast Red TR Salt
None
2 types of Hematoxylin stain that can be used for IHC
A type of hematoxylin stain that produces reddish violet stain
Mayers
A type of hematoxylin stain that produces red stain; exhibit tissue morphology well
Harris
Known as Traditional counterstain, nuclear stain
Hematoxylin