Introduction to IHC

Question 1

Immunohistochemistry test is used for

Answer 1

1. Identify tissue/cellular antigen or phenotypic marker
2. Detect microbes that causes disease in cytological preparation

Question 2

Give 3 cytological preparations

Answer 2

• Sputum
• Fluids
• Fine needle aspirations

Question 3

IHC involves what kind of reaction

Answer 3

Antibody-antigen Reaction

Question 4

T/F. IHC is a routinely used test

Answer 4

False ; special test

Question 5

IHC uses what substances as an indicator

Answer 5

Antibodies/immunoglobulins OR Lectins

Question 6

Most common antibody used in IHC

Answer 6

IgG

Question 7

Give 2 Types of Antibodies used in IHC

Answer 7

1. Polyclonal
2. Monoclonal

Question 8

A type of antibody that is being produced by different cells

Answer 8

Polyclonal

Question 9

T/F. Polyclonal reacts with various epitopes

Answer 9

True

Question 10

A part of an antigen that reacts with antibody

Answer 10

Epitope

Question 11

Polyclonal: Laboratory animal sources

Answer 11

Goat
Sheep
Pig

Question 12

A type of antibody that is being produced by individual clone of plasma cell

Answer 12

Monoclonal

Question 13

Monoclonal antibody only reacts with

Answer 13

Only one specific epitope

Question 14

A type of antibody that is more specific

Answer 14

Monoclonal

Question 15

Main source of Monoclonal antibody

Answer 15

Mice