Common Mistakes!

Question 1

Function of each:
1. Neutrophil
2. Eosinophil
3. Monocyte
4. T-Lymphocytes (in peripheral blood)
5. B-Lymphocytes (in lymph nodes)

Hint: Don’t say “apart of inflammation”

Answer 1

1. Phagocytize & destroy foreign particles with chemicals such as H2O2.
2. Fight parasites & lead allergic reactions.
3. Phagocytize foreign material & present antigen to lymphocytes.
   - Lymphs can’t function without them!
   - Mono’s in tissue are called Macrophages
4. Cellular immunity.
   - Against infected cells or cancer.
5. Produce antibodies.

Question 2

Features of malignancy (5)

Answer 2

1. High N:C ratio
   - Large nucleus to Small cytoplasm.
2. Mitotic figures
   - Cells beginning or in the midst of mitosis.
3. Multiple nucleoli in nuclei
4. Bizarre variable nuclei in cell population with open chromatin
5. Anisocytosis

Question 3

Features of inflammation (2)

Answer 3

1. Presence of neutrophils, eosinophils, or macrophages in sample at numbers greater than peripheral blood.
2. Infectious organisms present.
   - Especially inside of neutrophils or macrophages.

Question 4

Features of Regenerative anemia (4)

Answer 4

1. Nucleated RBCs
   - Immature RBCs still containing a nucleus.
2. Reticulocytes
   - Larger & bluer on diff quik. Dots on new methylene blue.
3. Polychromasia
4. Anisocytosis.

And NOTHING else!

Question 5

Unit for total protein
8.0____

Answer 5

8.0gm/dL

Report to the tenths!

Question 6

Unit used when doing CBC
26,000____

Answer 6

26,000wbc/uL

”/” needs to be included for numbers per volume!

Question 7

T/F: In regard to urine sediment, specifying Calcium oxalate monohydrate & Calcium oxalate dihydrate isn’t necessary.

Answer 7

False!
Monohydrate and Dihydrate are different and require dramatically different care!

• Monohydrate = ethylene glycol poisoning
• Dihydrate = breed related diet processing

Question 8

1. What is the average size of a urine cast?
2. What objective could they be seen on?

Answer 8

1. At least two RBCs wide and 4-20 RBCs long.
2. Easily on LPF

Question 9

What are “toxic” lymphocytes?

Answer 9

Trick question, there’s no such thing!

REACTIVE lymphocytes might be what you’re thinking of.

Question 10

Reactive lymphocytes are a sign of what?

Answer 10

Inflammation
- Not malignancy

Question 11

What causes cell clusters?

Answer 11

Sample technique
- In tissue all cells stick together.