Hemoglobin is measured indirectly by converting it into? (4)
- Cyanmethemoglobin
- Acid Hematin
- Alkali Hematin
- Carboxyhemoglobin
Cyanmethemoglobin Method is also known as?
Hemoglobin Cyanide (HiCN)
Advantages of Cyanmethemoglobin (4)
- Stable in Dilution
- Commercially Available
- All forms except Sulfhemoglobin
- Allow different types of spectrophotometer
Drabkin Solution Composition (4)
- Potassium Ferricyanide
- Potassium Cyanide
- Sodium Bicarbonate
- Surfactant
Principle of Cyanmethemoglobin Method
Hemoglobin is Oxidized from Ferrous to ferric state by Potassium Ferricyanide to form Methemoglobin. Then Potassium Cyanide converts methemoglobin to Cyanmethemoglobin
pH for Sodium Bicarbonate substitute
7.0-7.4
What does substituting Sodium Bicarbonate do to the Hemoglobin?
shortens time for conversion from 10 mins to 3 mins
What is the wavelength needed for spectrophotometer in Cyanmethemoglobin Method?
540 nm
The absorbance is directly _____ to the ______
Proportional / Hemoglobin Concentration
How many minutes do you stand the solution for?
10 mins
3 Sources of Error for Cyanmethemoglobin Method
- Reagent is Light Sensitive
- Reagent is Highly Toxic
- Time Consuming and Erroneous delivery Results due to Heavy Smoker
Turbidity will cause?
False-Increase Result
High WBC Count?
> 20x10^9/L
High Platelet Count?
> 700x10^9/L
Remedy for High Platelet Count?
Reagent and Specimen should be Centrifuged and Supernatant is measured
Remedy for Lipemic Patient
Add 0.01 ml of plasma to 5ml of Reagent to use as blank
Remedy for Hemoglobin S and Hemoglobin C
1:2 Dilution with distilled water (1 Part Specimen 1 Part water) then multiply result by 2
Abnormal Globulins can be found in patients with? (2)
- Plasma Cell Myeloma
- Waldenstrom Microglobulinemia
Remedy for Abnormal Globulin?
Add 0.1g of Potassium Carbonate to Reagent
Reference Values for Cyanmethemoglobin Method
Male: 14-18 g/dl
Female: 12-15 g/dl
