RBC Count

Question 1

Number of Erythrocytes contained in 1 liter of blood

Answer 1

Erythrocyte Number Concentration

Question 2

Erythrocyte Number Concentration is expressed as?

Answer 2

Number of Cells per Cubic Millimeter

Question 3

RBC Count can be used to help diagnose? (2)

Answer 3

1. Anemia
2. Polycythemia

Question 4

Manual Methods to Count RBC

Answer 4

1. Microscopic
2. Unopette System
3. Tube Dilution

Question 5

RBC Pipette can aspirate up to?

Answer 5

0.5 - 1.0

Question 6

Dilution Factor of RBC Pipette

Answer 6

200

Question 7

Why is EDTA used for RBC Counting?

Answer 7

Retains Cell Morphology

Question 8

How many squares are counted in RBC Count?

Answer 8

5 Tertiary Squares

Question 9

Characteristics of Good RBC Diluting Fluid (7)

Answer 9

1. Isotonic Solution
2. High SG
3. Easy to Prep
4. Cheap
5. Good Preservative
6. Buffer Action
7. Not initiate growth of molds

Question 10

RBC Diluting Fluids (7)

Answer 10

1. Dacie’s
2. Hayem’s
3. Gower’s
4. Toisson’s
5. NSS
6. Bethell’s
7. 3.8% Sodium Citrate

Question 11

Best RBC Diluting fluid and can be stored for long periods of time

Answer 11

Dacie’s / Formol Citrate

Question 12

Best RBC Diluting fluid and can be stored for long periods of time

Answer 12

Dacie’s / Formol Citrate

Question 13

This Diluting Fluid allows growth of yeast, Clumping of cells and has no corrosive effect

Answer 13

Hayem’s

Question 14

This diluting fluid prevents rouleaux formation and precipitates protein

Answer 14

Gower’s

Question 15

Diluting fluid that support growth of fungi with High SG and can also stain WBCs

Answer 15

Toisson’s

Question 16

Used in emergency cases and can cause excessive rouleaux formation

Answer 16

NSS

Question 17

Angle of Pipette while charging?

Answer 17

30-35 Degrees

Question 18

Stand the Counting chamber for how many minutes?

Answer 18

5-10 mins

Question 19

Reference Range for RBC Count?

Answer 19

Male: 4.5-6 M/cumm
Female: 4.0-5.5 M/cumm
Late Pregnancy: 3-5 M/cumm
At Birth: 7.0 M/cumm

Question 20

3 Errors due to Materials and Equipment

Answer 20

1. Debris in coverslip/chamber
2. Old Diluting Fluid
3. RBC Pipette not clean

Question 21

6 Errors due to Nature/Operators error

Answer 21

1. Incorrect Dilution
2. Did not Discard
3. Overcharging
4. Undercharging
5. Air bubbles
6. Incorrect Counting

Question 22

If RBC is markedly increased?

Answer 22

Decrease Volume of Aspirated Blood

Question 23

If RBC is markedly decreased?

Answer 23

Increase Volume of Aspirated Blood

Question 24

What may result to increased RBC Count? (2)

Answer 24

1. Dehydration
2. Strenuous exercise Activities

Question 25

5 Pathologic Variations of RBC Count?

Answer 25

1. Polycythemia 2. Anemia 3. Hemorrhage 4. Drugs 5. Secondary to other diseases

Question 26

4 Types of Counting Chamber

Answer 26

1. Improved Neubauer 2. Neubauer 3. Fuchs-Rosenthal 4. Speirs-Levy

Question 27

Depth of Improved Neubauer

Answer 27

0.1 mm