D1.1 DNA replication

Question 1

what processes require DNA replication?

Answer 1

Reproduction, growth and repair

Question 2

What does it mean to copy DNA faithfully?

Answer 2

copying without mistakes

Question 3

what do we mean by semi-conservative

Answer 3

contains 1 strand from parent and 1 strand from new

Question 4

what is the role of DNA polymerase in DNA replication?

Answer 4

• adds nucleotide to single-stranded
  region
• ensures correct complementary
  bonding, ensuring hydrogen forms
  between them

Question 5

what is the role of Helicase as a replication enzyme?

Answer 5

unwinds the double helix at replication forks by breaking down hydrogen bonds

Question 6

what is the role of Primase as a replication enzyme?

Answer 6

making and synthesizing RNA primers

Question 7

what is the role of DNA polymerase III as a replication enzyme?

Answer 7

synthesizes new strand by adding nucleotide to 3’ end onto the primer (5’ to 3’ direction). DNA polymerase III removes any nucleotide from the 3’ terminal

Question 8

what is the role of DNA polymerase I as a replication enzyme?

Answer 8

Removes the primer and replaces it with DNA allowing DNA polymerase I to attach

Question 9

ALWAYS 3’ end

Answer 9

what end are nucleotides always added to

Question 10

What is an Okazaki fragment?

Answer 10

short DNA segments synthesized discontinuously on the lagging strand during DNA replication

Question 11

what is the difference between continuous and discontinuous synthesis?

Answer 11

Continuous synthesis requires one primer, whereas discontinuous requires multiple primers and ligase to join segments

Question 12

darme los pasos de DNA replicación

Answer 12

buscas tus notas y dibujas