Diagnostic Testing

Question 1

Describe sensitivity VS specificity.

Answer 1

Sensitivity:
-measures capacity of test to correctly identify the true positive (sick)
Specificity:
-measures capacity of test to correctly identify the true negative (healthy)

Question 2

Describe direct serology.

Answer 2

-presence of antigen in sample
-positive result = infection

Question 3

Describe indirect serology.

Answer 3

-detect presence of antibodies in sample
-positive result = exposure

Question 4

Describe ELISA.

Answer 4

-uses enzymes labeled antibodies/antigens to detect/measure concentration of an analyte

Question 5

Direct Elisa.

Answer 5

Question 6

Describe indirect Elisa.

Answer 6

1. Well is pre coated with antigen.
2. Test serum is added - antibodies bind.
3. Labeled antiglobulin is added.
4. Enzyme substrate is added.
5. Color changes.