EXERCISE 2

Question 1

importance of MICROBIOLOGICAL EXAMINATION OF WORKING SURFACES

Answer 1

determine cross contamination,
Validation and monitoring of cleaning and disinfection protocols

Question 2

two main methods of MICROBIOLOGICAL EXAMINATION OF WORKING SURFACES

Answer 2

. Nonmicrobiological
2. Microbiological

Question 3

Characteristics of an ideal assessment method:

Answer 3

1. Sufficient sensitivity
2. Works well on both wet and dry surfaces
3. Good repeatability and reproducibility
4. Easy to use, rapid, and cheap

Question 4

difference between repeatability and reproducibility

Answer 4

Repeatability: if you can produce consistent result within laboratory
Reproducibility: if you can produce consistent results among laboratories

Question 5

Different factors that could affect the occurrence of contaminants in ready-to-eat food

Answer 5

• raw mats, people, processing equip, processing envt, animals, pests,

Question 6

compare VISUAL, MICRO, AND ATP TESTING

Question 7

ATP bioluminiscence principle

Answer 7

Amount of light is directly proportional to the amount of ATP on a surface

Question 8

device used in ATP bio

Answer 8

luminometer

Question 9

standard limit for ATP biolumi

Answer 9

<500 RLUs

Question 10

direct method is also known as

Answer 10

replicate organism direct area contact (RODAC)