EXERCISE 4

Question 1

Danger to public health of not having WP

Answer 1

1. Risk of anaphylactic reaction
2. Aplastic anemia, granulocytopenia (low granulocyte count), optic neuritis (inflamm of optic n.) and gray symptom in newborn infants
3. Gut microflora is altered.
4. AMR

Question 2

Commonly used organisms in antibiotic residue

Answer 2

1. Sarcina lutea
2. Bacillus subtilis
3. Bacillus cereus var. Mycoides
4. Micrococcus flavus

Question 3

what antibiotic do sarcina lutea detect?

Answer 3

PCS

Question 4

what antibiotic do b. subtilis detect?

Answer 4

streptomycin

Question 5

what antibiotic do b. cereus var. mycoides detect?

Answer 5

tetracycline

Question 6

what antibiotic do micrococcus flavus detect?

Answer 6

bacitracin

Question 7

a non-selective and non-differential medium
Almost similar sa nutrient agar

Answer 7

Mueller Hinton Agar

Question 8

factors affecting ZOB

Answer 8

1. bacterial conc
2. agar choice
3. depth, pH
4. test bacterium

Question 9

standard bacterial concentration inoculated

Answer 9

0.5 McFarland capacity

Question 10

amounts of ______ can also affect ZOB

Answer 10

thymine, thymidine, PABA

Question 11

max incubation time

Answer 11

16-24 hrs