export_exam 3

Question 1

Adenine (name of nucleoside and nucleotide)

Answer 1

1. Nucleoside: adenosine 2. Nucleotide: adenylate

Question 2

Guanine (name of nucleoside and nucleotide)

Answer 2

1. Nucleoside: guanosine 2. Nucleotide: guanylate

Question 3

What are the purines?

Answer 3

Adenine and guanine

Question 4

What are the pyrimidines?

Answer 4

Cytosine, thymine and uracil

Question 5

Cytosine (name of nucleoside and nucleotide)

Answer 5

1. Nucleoside: cytidine 2. Nucleotide: cytidylate

Question 6

Thymine (name of nucleoside and nucleotide)

Answer 6

1. Nucleoside: Thymidine 2. Nucleotide: Thymidylate

Question 7

Uracil (name of nucleoside and nucleotide)

Answer 7

1. Nucleoside: uridine 2. Nucleotide: uridylate

Question 8

A codon consists of how many nucleotides? How many codons are there?

Answer 8

* 3 * 64

Question 9

Sanger sequencing depends on the use of what which cause termination of the chain. The traditional method requires how many separations?

Answer 9

* Dideoxynucleotide * 4

Question 10

In solid phase synthesis 1. what OH is attached to the silica bead 2. H phosphonate/phosphoramidite require phosphorous to be 3. what base does not need to be protected

Answer 10

1. 3’ 2. Oxidized 3. Thymine

Question 11

DNA polymerases such as those used in Sanger sequencing and PCR require the nucleotide in the form of what . Sanger sequencing and PCR also start with a short oligonucleotide oligomer called a what.

Answer 11

* deoxynucleotide triphosphate * primer

Question 12

Nucleic acids can be purified by what -exchange chromatography

Answer 12

Anion

Question 13

RNA is less stable in basic conditions than DNA because of the

Answer 13

2’-OH