1
Q
Cation exchanger
A
negative resin binds positive proteins
2
Q
Anion exchanger
A
positive resin binds negative proteins
3
Q
pH > pI
A
protein negative
4
Q
pH < pI
A
protein positive
5
Q
Gradient elution
A
increase salt → proteins elute according to charge
6
Q
Counter ions
A
Na⁺ or Cl⁻ that compete to displace proteins
7
Q
Vo
A
void volume (large proteins elute here first)
8
Q
Vt
A
total column volume
9
Q
Exclusion limit
A
above this MW, proteins can’t enter pores
10
Q
Fractionation range
A
MW range that separates well
11
Q
SDS gives proteins uniform
A
negative charge
12
Q
Proteins migrate to
A
anode
13
Q
Separation based on
A
size only
14
Q
β-mercaptoethanol breaks
A
disulfide bonds
Biochemistry Lab
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