1
Q
Why does salt elute proteins in ion exchange?
A
Salt ions (Na⁺ or Cl⁻) compete with proteins for charged sites on the resin, weakening interactions and releasing bound proteins.
2
Q
Why does ammonium sulfate precipitate proteins?
A
It ties up water molecules, reducing solvation of proteins, causing hydrophobic patches to aggregate.
3
Q
Why SDS-PAGE separates by size?
A
SDS denatures proteins and coats them with uniform negative charge, eliminating charge differences.
4
Q
Why proteins move to anode
A
Because SDS makes them strongly negative
5
Q
How proteins elute in gel filtration?
A
Large proteins elute first (Vo), small proteins elute last (enter pores)
6
Q
A
Biochemistry Lab
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