Formative test Pipetting

Question 1

The best pipet to use when SEPARATING serum or plasma
from other blood components is the

*volumetric pipet
*serologic pipet
*disposable pipet or Pasteur pipet
*micropipet

Answer 1

disposable pipet or Pasteur pipet

Question 2

In operating semiautomated
micropipettes, which of these steps is NOT NECESSARY?

*wiping the immersed tip

*repetitive depression of the plunger button to 2nd stop position

*checking the fitting of the tip to the pipet

*putting the plunger to 1st stop position

Answer 2

wiping the immersed tip

Question 3

Which of the following pipetting steps require SLOW & SMOOTH
operation?

Answer 3

Wiping the immerse tip with absorbent

Question 4

The FIRST STEP in transferring
fluids with the use of micropipets is

Answer 4

Attachment of the tip

Question 5

What are the DESIGNS of pipettes?

Answer 5

To deliver (TD) & to contain (TC) types

Question 6

Give the MECHANISM of pipetting that occurs inside an
AUTOMATED CHEMISTRY ANALYZER.

Answer 6

• sample identifications
• aspiration
• dispensing
  -mixing and reaction
  -washing
  -calibration and quality control
  -disposal

Question 7

Which accessory device is NOT ATTACHED to a semiautomated
micropipette?

Answer 7

disposable tips

Question 8

Which of the following is NOT A
PART of a volumetric pipet?

Answer 8

white rings

Question 9

Enumerate 5 DISEASES
contributed by performing pipetting for many years or
brought about by erroneous longterm pipetting techniques.

Answer 9

• Carpal tunnel syndrome
  -tendinitis
  -musculoskeletal
• eyestrain

Question 10

Which FINGER is best suited for the regulation of fluid flow inside the glass volumetric and
graduated pipets?

Answer 10

index finger
forefinger

Question 11

In operating semiautomated micropipettes, which of these steps is NOT ALLOWED?

• holding the pipette in an inclined position
• reusing the attached
  tips several
  times
• using the index finger to eject the tips off the pipette

-using the same tip many times in
transferring the same reagent into many tubes

Answer 11

• using the index finger to eject the tips off the pipette

Question 12

Which type of PIPETTE is NOT equipped with calibration mark or marks

Answer 12

• Sahli micropipette
• Volumetric pipets
• Serologic pipettes
• Mohr pipettes
• Pasteur pipettes
• Disposable pipettes
• Medicine droppers

Question 13

Which pipet is manipulated NOT following the AIR DISPLACEMENT principle?

• disposable polyethylene pipets
• volumetric pipets
• graduated pipets
• micropipets
• pasteur pipets
• medicine droppers
  *none of these

Answer 13

*none of these

Question 14

When pipetting SERUM SAMPLES, * it should be noted to observe
which of the following steps?