Gluconeogenesis

Question 1

what is gluconeogenesis

Answer 1

making glucose from pyruvate with 3 bypass steps

Question 2

is gluconeogenesis exothermic or endothermic?

Answer 2

highly endothermic

Question 3

how many enzymes catalyze gluconeogenesis?

Answer 3

2, carboxylase then kinase

Question 4

carboxylase

Answer 4

c-c bond forming

ATP activates CO2 for Nu attack

Question 5

step 1

Answer 5

enzyme name: carboxylase

enzyme cofactor: biotin

pyruvate + HCO3- + ATP –> oxaloacetate + ADP + Pi

Question 6

step 2

Answer 6

enzyme name: kinase

oxaloacetate + GTP —> PEP + CO2 + GDP

Question 7

driving force of step 2?

Answer 7

charge charge repulsion of Asp or Glu in active site of enzyme next to - charge of oxaloacetate

causes CO2 formation