HMP

Question 1

glucose 6P DH

Answer 1

uses NADP+ and H2O to make NADPH and 6-phosphogluconolactose

inhibited by NADPH

Question 2

Cell needs NADPH on HMP

Answer 2

@FA syn and detox

oxidative rxns produce NADPH
-nonoxidative rxns convert ribulose 5P to glucose 6P to produce more NADPH

use both pathways

Question 3

cell needs NADPH and pyruvate on HMP

Answer 3

@RBCs Oxidative and non oxidative rxns used

• oxidative gen NADH ade ribulose 5P
• nonox convert ribulose 5P to fructose 6P and glyceraldehyde 3P which glycolysis converts to pyruvate

use both pathways

Question 4

Pathways requiring NADPH

Answer 4

Reductive biosyn

cytochrome p450 monooxygenase system

phagocytosis by WBC

Syn of NO

Reduction of H2O2 (ROS)

Question 5

CYP450 activity

Answer 5

1. Add OH group to make toxins/drugs more soluble
2. Mitochondrial-biosyn of steroid hormones
3. Mitochondrial-Liver-Biosyn of bile and active vit D3

Needs NADPH and Oxygen to function

Question 6

Glutathione reduction

Answer 6

Glutathione in reduced form=GSH

OHe- to H2O with glutathione

• after reduces to H2O=becomes oxidized and inactive by glutathione reductase
• NADPH re-reduces glutathione using glutathione reductase

Question 7

Superoxide reaction

Answer 7

Hb-Fe2+O2 to Hb-Fe3+ + O2e-
Spontaneous
Occurs in highly oxidizing environemtnof RBC
Why GSH is important

Question 8

Glucose 6P DH def what it does and then what occurs (4 things)

Answer 8

won’t make NADPH from glucose 6P (also can’t do HMP pathway)

• ROS accumulate because no GSH re-reduction
• Heinz bodies (precipitation of Hb) are symptomatic
• Worsened by oxidant stress (infections/drugs/fava beans)
• hemolysis by ROS

Question 9

Favism-Alternate name?

• inheritance
• homo vs hetero?
• why occur?
• Sx

Answer 9

Glucose 6P DH def
-x link recessive
hetero is usually asymptomatic if no oxidant stress
-offers relative resistance to type of malaria

Sx-Heinz bodies, anemia

Question 10

Malic enzyme

Answer 10

in Malate to pyruvate in lipid synthesizing cells (adipose, mammary gland, liver)

produces 70% total NADPH

Question 11

Nicotinamide Nucleotide Transhydrogenase

Answer 11

NADH to NADPH (and NAD+ obviously)

Close to glutathione reductase so can product vs ROS coming off ETC from CoQ