TEM
50pm resolution, uses electrons and magnets
SEM
Used for looking at surface of cells. 1 nm resolution
Red blood cell size
7.8 um
Artifacts
Issues introduced to slides
Histochemistry
Stains reaction product, showing enzyme activity
Immunolocalization
Antibodies can find specific proteins in sections
Freeze fracture
Distribution of proteins in membranes.
Negative staining TEM
Visualize individual proteins isolated from cell
Autoradiography
Label metabolic processes
FISH
DNA probes localize genes