Kirby-Bauer Disk Diffusion Test

Question 1

Kirby-Bauer Disk Diffusion Test

Answer 1

• all labs that do this test must follow same standards

- Standardized procedure for determining bacterial resistance

Question 2

Media Used for Kirby-Bauer Disk Diffusion Test

Answer 2

Mueller- Hinton Agar (must be 4 mm deep)

Question 3

The time plates are incubated for Kirby-Bauer Disk Diffusion Test

Answer 3

18-24 hours

Question 4

Temperature of incubation for Kirby-Bauer Disk Diffusion Test

Answer 4

35 +- 2 Degrees C

Question 5

Amount of Bacteria needed for Kirby-Bauer Disk Diffusion Test

Answer 5

0.5 McFarland Turbidity Standard

150 million bacterial cells per mL of liquid

Question 6

Age of Culture for set up for Kirby-Bauer Disk Diffusion Test

Answer 6

2-6 hours old

Question 7

Mechanism of Drug Action: How Antibiotics Work

Answer 7

• inhibition of cell wall synthesis
• inhibition of protein synthesis
• inhibition of structures and functions of DNA + RNA
• blocks a key metabolic pathway
• breakdown of the cell membrane function or structures