L7: Cell Models

Question 1

New medicines need not be tested in animals to receive U.S. Food and drug administration (FDA) approval as announced in 2023

True or false

Answer 1

True

Question 2

Physiological barriers for oral drug delivery does NOT include:

Biochemical agents in the stomach and intestine lumen

Mucus layer lining the whole GI tract

The circulatory system

The epithelium

Answer 2

The circulatory system

Question 3

Major cellular components in the small intestinal epithelium include:

Answer 3

Enterocytes
Goblet cells
Microfold cells (M-cells)
Smooth muscle cells

Question 4

Coco-2- cells have been considered the “gold standard” assay to predict intestinal absorption, due to their ability of

Answer 4

Forming cell layers with functional tight junctions

Question 5

HT29-MX is a cell line typically used for mimicking goblet cells in secreting gel-forming mucins
True or false

Answer 5

True

Question 6

With HT29-MTX cells it is possible to replicate the whole thickness of in vivo mucus layers

True or false

Answer 6

False

Question 7

Microfold cells (M- cells) are needed if mucosal immunity response is involved in the study:

True or false?

Answer 7

True

Question 8

Monocytes can be included in an in vitro cell model if the study aims for investigating
1. Cellular uptake of drugs
2. Drug transportation
3. Inflamed epithelium conditions
4. Drug penetration through mucus

Answer 8

Inflamed epithelium conditions

Question 9

In a transwell plate based cell model, cells can only be seeded in the apical side, while the bottom chamber should leave blank,

True or false

Answer 9

False

Question 10

TEER value measurement is a useful approach for investigating barrier integrity of in vitro epithelium models. Here, TEER is an abbreviation of:

Answer 10

Trans- epithelial electrical resistance

Question 11

Immunofluorescence imaging of expressed ZO-1 protein is a useful characterization method to investigate barrier integrity of in vitro epithelium model

True or false

Answer 11

True

Question 12

Which of the following in vitro model can provide better mimicry of the 3D villi -like structure?

• Caco- 2- cells on 96 well plate
• Caco-2- cells on transwell plate
• Gut on a chip
• Co- culture of Caco- 2 and HT29-MTX cells on transwell plate

Answer 12

Gut on a chip

Question 13

Unlike transwell plate model, a gut on a chip model is special because it provides:

Answer 13

Dynamic flow

Question 14

As a senior scientist in a big Pharma company, you are looking for a reliable in vitro model for drug testing. Which company will you reach out to?

Answer 14

Celloop. - underviseren arbejder her