lab 4 Flashcards

(30 cards)

1
Q

What is the ideal temperature for bacterial growth called?

A

Optimum

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2
Q

What is the lowest temperature at which bacteria can grow?

A

Minimum

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3
Q

What is the highest temperature at which bacteria can grow?

A

Maximum

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4
Q

True or False: You’re killing microbes when just a little too hot or a little too cold.

A

False

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5
Q

What are the bacteria that only grow below 20°C called?

A

Psychrophiles

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6
Q

What type of bacteria can grow at cold temperatures but survives up to 35°C?

A

Psychrotrophs

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7
Q

What is the temperature range for Mesophiles?

A

15°C-45°C

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8
Q

What type of bacteria grows at temperatures of 45°C-80°C?

A

Thermophiles

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9
Q

What are bacteria that survive from 80°C-110°C called?

A

Extreme thermophiles

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10
Q

What are Mesophiles that can survive at high temperatures (70°C or higher) called?

A

Thermoduric

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11
Q

Define Turgor Pressure.

A

How hard a cell pushes out into its environment

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12
Q

What drives osmosis?

A

Solute concentration

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13
Q

What is the term for when solute concentrations are equal on both sides of a cell membrane?

A

Isotonic

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14
Q

Fill in the blank: In a _______ solution, water will flow into the cell, and it will swell up.

A

Hypotonic

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15
Q

What happens to a cell in a hypertonic solution?

A

Water leaves cell, causing plasmolysis

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16
Q

What is the classification for bacteria that survive low salt concentrations (0-5%)?

17
Q

What type of bacteria can grow in moderate salt concentrations (0%-11%)?

18
Q

What are bacteria that can grow ONLY in high salt concentrations (5-15%) called?

19
Q

What classification is given to bacteria that can grow in HIGH salt concentrations (15-30%)?

A

Extreme Halophiles

20
Q

What type of bacteria can tolerate very high sugar concentrations?

21
Q

What are the classifications for oxygen requirements?

A
  • Strict aerobes
  • Facultative anaerobes
  • Aerotolerant
  • Microaerophilic
  • Strict anaerobes
22
Q

What do strict aerobes need to survive?

23
Q

Fill in the blank: Facultative anaerobes can use oxygen but _______ need it.

24
Q

What is the main component of the cell walls in acid-fast bacteria?

25
What color indicates an acid-fast positive result?
Pink
26
What color indicates an acid-fast negative result?
Blue
27
What is the first step in the acid-fast stain procedure?
Prepare heat fixed smear of bacteria
28
How long should carbolfuchsin be left on the smear during the acid-fast stain?
At least 5 minutes
29
What is used to decolorize the smear in the acid-fast stain?
Acid alcohol
30
What is the counter stain used in the acid-fast staining procedure?
Methylene blue