lab 8

Question 1

Solution II containing sodium dodecylsulfate (SDS) and sodium hydroxide (NaOH)

Answer 1

SDS is a detergent that denatures proteins and disrupts the plasma membrane causing the cell to lyse(released into solution)
NaOH increase pH of the lysate and denatures the DNA
- Genomic DNA strands are able to move a greater distance

Question 2

Solution III (acidic potassium acetate solution)

Answer 2

Causes some of the hydrogen bonds in the genomic DNA to re-form

• random pairing of complementary bases to create a tangled, insoluble mass of DNA
• acts as the salt in the banana DNA extraction

Question 3

Ethanol in the supernatant solution

Answer 3

Removes water molecules from DNA

- the sodium ions and ethanol cause the plasmid DNA and RNA to come out of solution and precipitate

Question 4

Solution T ( Tris buffer)

Answer 4

Any residual DNAese left in the preparation would be able to degrade the plasmid DNA

Question 5

Bacterial genomic DNA

Answer 5

Approx 4000 genes in total

• arranged in a circle that is anchored to the bacterial plasm membrane
• kind of looks like squiggles

Question 6

Bacterial plasmid DNA

Answer 6

In some bacterial cells
Usual small
Float freely in cell’s cytoplasm
- supercooled conformation