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lab safety & math Flashcards

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1
Q

glassware that has critical measurement

A
  • volumetric flask
  • volumetric pipette
  • serological pipette
  • MoHR pipette
  • micropipette
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2
Q

level III (3) water -
made how?
used for?
used as?

A
  • made thru activated charcoal filter (brita)
  • used for washing/rinsing glassware
  • used as base for making level I and II water
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3
Q

purified, practical or commercial grade used where

A

in manufacturing

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4
Q

what is LSCCLA responsible for? (3)

A
  • billing and collection of specimens appropriate to both hospitals and private labs
  • regulate who can order lab tests
  • provides license that allows hospitals or private lab to operate
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5
Q

types of reusable glass in lab

A
  • borosilicate

- quartz (clear)

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6
Q

Level I (1) water
purity?
made how?
iportant info?

A
  • purest
  • made by deionizing AND distillation
  • must be made as needed - do NOT store
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7
Q

how does a hot air oven sterilize

A

160 degrees Celsius for 1 hour continuously

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8
Q

UN 2900

A

infectious substance affecting animals only

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9
Q

united stated pharmacopeia/national formulary grade used where

A

in pharmacies

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10
Q

UN 3373

A

biological substance (category B)

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11
Q

PHIPA

A

Personal Health Information Protection Act

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12
Q

LSCCLA

A

Laboratory and Specimen Collection Centre Licensing Act

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13
Q

Class I (1) bio safety cabinet

  • appearance
  • exhaust where
  • hepa filter
  • where used
A
  • open appearance
  • vents into room or externally
  • 1 hepa fitler
  • used in pharmacy
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14
Q

KHDBDCM

A 
k-kilo
h-hecto
d-(da)deca
b-(base)gram/litre/meter
d-(d)deci
c-centi
m-milli
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15
Q

how is the MEDIAN calculated

A

central or middle value in numerical order

  • odd numbers-middle is median
  • even numbers-middle 2 numbers added together then divide by 2 is median
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16
Q

LPTP

A

laboratory proficiency testing program

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17
Q 
class II (2) biosafety cabinet
-appearance
-exhaust where
-hepa filter
used where
A
  • partial opening with 8-10” glass sash
  • vents into room or externally
  • 2 hepa filters
  • used in routine labs
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18
Q

sanitization examples (5)

A
  • 70% isopropyl alcohol
  • mild detergent
  • soap
  • alcohol based hand rubs
  • heavy organic debris present - sanitize then disinfect
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19
Q

transfer pipettes - critical? graduated?

A
  • non critical

- may be graduated

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20
Q

sanitization - surface type

A

both animate and inanimate

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21
Q

sterilization purpose

A

destroy all microorganisms and their spores

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22
Q

sterilization - surface type

A

inanimate objects

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23
Q

serological pipettes - critical? graduated?

A
  • critical

- graduated yes (measurement ends at tip)

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24
Q

bottles - critical? graduated?

A
  • non critical

- may be graduated

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25
pasteure pipettes - critical? graduated?
- non critical | - may be graduated
26
describe a beaker in terms of appearance and use
- wide mouthed - straight sided - pour spout - markings to indicate volume
27
use for a beaker
mixing bowls for labs
28
class III (3) biosafety cabinet - appearance? - exhaust where? - hepa filter? - used when?
- closed with glove box - vents externally ONLY - minimum 2 hepa filters - usually 3 - used for high risk (anthrax, TB, ebola
29
microbiology sub-departments
- bacteriology - virology - parasitology - mycology
30
where do graduations end on MoHR pipette
above the tip
31
reagent grade/analytical reagent grade - level of purity
highest degree of purity
32
UN 2814
infectious substance affecting humans
33
blow out
squeeze out last drop
34
where does category B box go
ALWAYS public health laboratory
35
UN 1845
dry ice/carbon dioxide inside
36
what glassware is used to measure 24-hour urine
graduated cylinder
37
type of disposable glass in lab
flint
38
technical grade - purity level
lowest purity
39
what does an ORANGE band indicate on a pipette
10ml
40
test tube - critical? graduated?
- non critical | - may be graduated
41
beakers - critical? graduated?
- non critical | - may be graduated
42
graduated cylinder - critical? graduated?
- non critical | - ALWAYS graduated
43
what does PHIPA govern
- regulate the sharing of information between HC providers and other organizations - sets standards for confidentiality in HC
44
UN 3291
clinical waste/medical waste
45
what is neutral pH
neutral pH is 7
46
pH above neutral
alkaline above 7
47
pH below neutral
acidic below 7
48
transfusion services sub-category
maternal/fetal compatibility
49
reusable plastic used in lab
polypropolyne
50
what is a micropipette used for
measure microliters
51
what does pH meter measure? | to determine what?
- measures potential hydrogen | - determine alkalinity or acidity
52
when sterilizing metal, auto clave must operate how
121 degrees Celcius for 15 minutes at 15psi
53
disinfection - surface type
inanimate
54
MoHR pipettes - critical? graduated?
- critical | - not graduated (measurements end above the tip)
55
Florence flask - critical? graduated?
- non critical | - NEVER graduated
56
volumetric flask - critical? graduated?
- critical | - NEVER graduated
57
Erlenmeyer flask - critical? graduated?
- non critical | - may be graduated
58
pH meter use
measure potential of hydrogen to determine alkalinity or acidity
59
who does PHIPA include (5)
- labs - doctors/hospitals - employers - schools - insurance companies
60
how is pH meter calibrated
kept moist in buffered solution of distilled water to maintain neutral pH
61
most common hematology test? | color tube?
- INR (international normalized ratio) | - light blue tube
62
to contain
rinse in final solution
63
how is the MEAN calculated
average of all values added together, then divided by number of values
64
how is SOLUTE CALCULATED
solute over solution % over 100 = x over solution
65
how is REAGENT equation calculated
part 1 reagent a over reagent a+b = x over total volume part 2 solute + solvent (x) = solution
66
what is LPTP responsible for
sends blind samples to determine proficiency in testing specimens
67
disposable plastic used in lab
polyethylene
68
level II (2) water purity made how used where
- mid purity - made by either deionizing or distillation - used in most routine lab procedures
69
what are we looking for when calculating the mode
precision
70
what are pH meter electrodes called
- reference/calomel probe | - glass probe
71
3 controls required with each use of autoclave
- temperature control - biohazard strip - chemical control
72
departments that blood specimens are sent to (5)
- hematology - clinical chemistry - microbiology - histopathology - transfusion services
73
reagent grade/analytical reagent grade used for
- research - producing controls - standards and buffers
74
disinfection purpose
reduce levels of microorganisms to a safe handling level
75
where do graduation end on serological pipette
end at the tip
76
united stated pharmacopeia/national formulary grade purity level
less pure than chemical pure
77
what does a BLUE band indicate on a pipette
5ml
78
vortex mixer use
tornado action to mix fluids in tubes (not good for delicate body fluids ex blood)
79
volumetric pipette - critical? graduated?
- critical | - never graduated
80
sanitization purpose
reduce levels of microorganisms to a safe handling level
81
micropipette - critical? graduated?
- critical | - never graduated
82
what does RED band indicate on a pipette
1ml
83
technical grade used where
industries
84
what is the goal of autoclave
kill all microorganisms and their spores
85
autoclave use
sterilizing metal equipment
86
hot air oven uses (3)
- incubate cultures - dry glassware and metal equipment - catalyst (enhances) and desiccants (absorbs moisture)
87
what does RHPA do (6)
- regulates professionals registered with college - protect public - professional accountability - prevents working outside scope - students are delegated - graduate deemed competent
88
what 2 things does the OHSA do
- sets out rights and requirements for employer and employee | - specifies workers right to health and safety
89
hematology sub-department
coagulation studies
90
how is the MODE calculated
most frequent occurring number (can have more than 1)
91
clinical chemistry sub-department
immunology
92
reagent grades - order of purity
1. reagent grade/analytical reagent grade 2. chemical pure 3. united stated pharmacopeia/national formulary grade 4. purified, practical or commercial grade 5. technical grade
93
chemical pure used for
laboratory purposes
94
histopathology sub-departments
- cytology | - cryogenics
95
uses for hot air oven
- incubate cultures - dry glassware - catalysts and desiccants - heat fix tissue to slide after warm bath
96
disinfection examples (6)
- 10% bleach - phenol products (lysol) - 70% iospropyl alcohol - 2% glutaraldehyde - Virkon - ammonia
97
antisepsis surface type
animate objects
98
purified, practical or commercial grade purity level
low purity rate
99
to deliver
retain last drop in pipette
100
incubator use
growing microorganism for 24 hour incubation period
101
antisepsis examples (7)
- alcohol based hand rub - 70% isopropyl alcohol - 3% hydrogen peroxide - chlorhexidine (not on babies) - tincture of iodine - paridone iodine - soap and water
102
antisepsis purpose
reduce levels of microorganisms to safe handling level to avoid spread of contagions
103
sterilization examples (5)
- autoclave - hot air oven - incineration - radiation - toxic gases
104
bacti-cinerator uses (2)
- heat fix smears | - sterilize inoculation loops
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