LAB TECHNIQUES

Question 1

disadvantages of haemocytometers

Answer 1

• dead cells look like live cells
• ## not very accurate numbers are estimates.

Question 2

What is the iso-electric point

Answer 2

the pH at which a soluble protein has no net charge and will precipitate out of solution

Question 3

WHat is the effect of buffers in iso-electric separation techniques

Answer 3

if solution is buffered to a specific pH, only the protein(s) that have an IEP of that pH will precipitate

Question 4

What is the charge on proteins at pH above their IEP

Answer 4

negstive

Question 5

What is the charge on proteins at pH below their IEP

Answer 5

positive