Buffered solutions reduce….
pH fluctuations
Adding an acid and conjugate base into a solo will be both a _______________ and a ________.
- specific pH
Henderson-Hasselbalch
used to determine pH of a given acid/base mix or correct mix to produce a desired pH
H+H equation
pH=pKa+log[conj.base]/[acid]
If pH is < pKa
-more acid
If pH is > pKa
-more basic
Both the amino and carboxyl ends of an amino acid can act as…
acid/conj.base pair
At low pH, both ends amino and carboxyl ends are…
protonated and charge is +1
At high pH, both ends amino and carboxyl ends are…
deprotonated and charge is -1