What is antigen detection
Demonstrate the organism
What is antibody detection
Indirect evidence of presence of organism
How to collect samples (2)
- Smears
2. Can extract nucleic acids from smears and histological sections and do PCR
How must blood be collected
Aseptically
How to pack blood
- Primary receptacle
- Protective 2nd layer
- Rigid outer packaging
What is a virus culture
Growth of live virus
What is serology
Indirect presence of virus - reaction of immune system - antibody
What are laboratory animas
Different animals are used for different microbes
What do embryonate chicken eggs help with
Different routes of infection - fast dividing
What are cell cultures
In vitro growth of animal/human cells
What are TCID50
Statistical value indicating the infectious nature of a virus. 50% tissue culture infected dose.
What is blood collected and put into (for virus isolation)
10ml in green-topped (heparin)
What is blood collected and put into (for serology isolation)
Collect in 10m in red-topped (plain)
What are signs cytopathic effect (4)
- Degeneration
- Syncytium
- Inclusion bodies
- Transformation
What is syncytium
A few host cells which merge cells
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