LECTURE 8 Flashcards

(81 cards)

1
Q

are prokaryotes a clade?

A

no

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2
Q

what defines bacteria?
they are called “prokaryotes” because:

A

because of what they lack :
- nuclei
- cellular organelles

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3
Q

prokaryotes are typically much __ than ____

A

much smaller than eukaryotes

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4
Q

most prokaryotes have: (3)

A
  1. cell walls
  2. circular chromosomes
  3. reproduce via binary fission
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5
Q

prokaryotes are immensly ___ and found ___

A

diverse and found everywhere

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6
Q

Prokaryotes are immensely diverse, and are found everywhere
* Incredibly numerous:

A

The estimated number of
prokaryotes in one handful of fertile
soil is greater than the number of
people who have ever lived

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7
Q

Prokaryotes are immensely diverse, and are found everywhere
Incredibly diverse:

A

there are more species of prokaryotes in 10 grams of soil than there are species of animals on earth

~7 million animal species, 1 to 100 million prokaryote species

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8
Q

How do we determine prokaryotic “species”?
(2)

A
  1. Prokayotes reproduce asexually
  2. Most species of prokaryotes are impossible to
    grow and observe in a lab
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9
Q

can we use the biological species concept to define prokaryotic species?

A

yes

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10
Q

We can use the ___
for prokaryotic species

A

ecological species concept

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11
Q

How do we determine prokaryotic “species”?
Metagenomics is
revealing clustering of genetic similarities in prokaryotes that: (2)

A
  1. We have classified as species
  2. Live in the same environment
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12
Q

All organisms are trying to solve the same basic problems (3)

A
  • Reproduction
  • Obtaining resources
  • Surviving current and future conditions (i.e. “avoid harm”)
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13
Q

how do prokaryotes avoid harm?

A

cell wall

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13
Q

nearly all prokaryotes have :
bacteria have:
archaea have:

A

CELL WALLS
Bacteria: peptidoglycan

Archaea: lack peptidoglycan; polysaccharides + proteins

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14
Q

the cell walls maintain ___ and ____

A

maintains structure and protects from outside

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15
Q

how do bacteria avoid harm other than cell wall?

A

CAPSULES

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16
Q

role of the capsule : (3)

A
  • Aids in cell adhesion to other cells,
  • protects against dehydration,
  • helps prevent attacks by the immune system
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17
Q

in many prokaryotes the capsule is:

A

Sticky layer of polysaccharides or proteins

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18
Q

how do bacteria avoid harm?

other than capsule or cell wall

A

endospores

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19
Q

the endospore preserves:

A

preserves the cell’s genetic material in times of extreme stress

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20
Q

__% of prokaryotes do not ___ under their own power
most of them:

A

50% of prokaryotes do not move under own power
they use a flagella

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21
Q

the flagella of ___, ___ and ___ have likely evolved ____

A

Flagella of bacteria,
archaea, and eukaryotes
likely evolved
independently

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22
Q

binary fission is:

A

reproduction without SEX

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23
Q

how is binary fission different from mitosis? (3)

A

No spindle, centrioles,
dance of the
chromosomes

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24
# how do prokaryotes adapt without sex mutations are ___ but bacteria are ___
rare but bacteria are numerous
25
bacteria evolve ___ to ___
rapidly to new conditions
26
how does genetic diversity arise is prokaryotes? (4)
rapid reproduction mutation horizontal gene transfer recombination
27
what is reprodcution without sex in bacteria
asexual binary fission
28
what is sex without reproduction in bacteria?
HGT
29
(4) genetic diversity in bacteria?
- genetic recombination - transduction - transformation - conjugation
30
what is vertical gene transfer
transmission of genetic material from parents to offspring during reproduction
31
what is horizontal gene transfer
(also called **lateral** gene transfer): Movement of genetic material other than by the transmission of DNA from parent to offspring. DNA is passed “**sideways**”
32
3 mobile genetic elements that make HORIZONTAL gene transfer possible?
- Plasmids (extrachromosomal genetic material) - Transposons (“jumping genes”) - Bacteriophages (bacteria-infecting viruses)
33
transposons:
jumping genes
34
extrachromosomal genetic material
plasmids
35
bacteriophages:
bacteria-infecting viruses
36
how are elements transferred between organisms in prokaryotes? (3)
In prokaryotes: - **transformation**, - **conjugation**, - **transduction** In eukaryotes? Much more complicated! Possible mechanisms: conjugation, **endocytosis**
37
what is conjugation
sex without reproduction genetic transfer involving 2 individuals through a pilus
38
# STEP 1 CONJUGATION a cell carrying an F plasmid forms ___ with ___ cell and a ___ breaks
mating bridge with F- cell (recipient) + a strand of the plasmid's DNA breaks
39
# step 2 conjugation the cell synthesizes a ___ from ___ as template the ____ and enters ___
cell makes a new strand from the unbroken one as a template the broken strand peels off and enters the F- recipient cell
40
# step 3 conjugation dna ___
DNA replication continues in both donnor and recipient cell
41
# step 4 conjugation once DNA transfer is complete :
new plasmid recircularizes and recipient cell is now recombinant F+ cell
42
what is transformation
the uptake of foreign DNA from the environment
43
**Transformation** Uptake of foreign DNA from environment Incorporated into:
into the host chromosomes through **recombination**
44
what is transduction
bacteriophages carry bacterial genes between hosts
45
steps of bacteriophage cycle
- phage infects bacteria - phage DNA replicates - new phage particles assemble and get packaged in a capsid - phage infects a new cell - donor DNA is incorporated through crossing over - result: genotype differs between donor and recipient cells
46
what are the oldest fossils?
stromatolites 3.5 billion BP
47
life began after : | what did it start with?
after earth formed and started with bacteria
48
what are stromatolites?
rock-like structures formed by bacteria theyre formed by cyanobacteria today
49
2 Sources of energy for all organisms?
Light: phototrophs Chemical bonds: chemotrophs
50
2 Sources of carbon?
Inorganic CO2: autotrophs Organic molecules: heterotrophs
51
ihow many of the 4 types of nutrtion do prokaryotes use?
4 types
52
chemoheterotrophs:
Most prokaryotes, animals, fungi, some protists
53
Only some prokaryotes (which nutrition mode?)
chemoautotrophs and photoheterotrophs
54
Plants, some protists, prokaryotes (cyanobacteria, purple sulfur bacteria, others) which nutrition mode?
PHOTO AUTO trophs
55
there is greater ___ in ___ than all ___
greater nutritional diversity in prokaryotes than in all eukaryotes combined
56
all forms of ___ evolved within prokaryotes
nutritional diversity
57
facultative **anaerobe**
some use oxygen but can do without it
58
which bacteria are poisoned by oxygen?
obligate anaerobes
59
which bacteria must use oxygen
obligate aerobes
60
what were the first prokaryotes
CHEMO HETERO trophs they consumed organic molecules anaerobic and did glycolysis
61
great oxygen event/catastrophe?
The evolution of photosynthesis led to an accumulation of oxygen in the atmosphere
62
name the 4 events leading to extinction of anaerobes
1. cyanobacteria 2. O2 accumulates 3. corrosive environment 4. extinction of anaerobes
63
final electron acceptor in cellular respiration?
oxygen
64
# 5 steps! hypothesized evolution of nutrient diversity?
1. first organisms: either chemoheterotrophs or chemoautotrophs (anaerobic) 2. anoxygenic photoautotrophs (anaerobic) 3. oxygenic photoautotrophs (anaerobic) 4. Chemical sinks saturate, oxygen increases 5. Aerobic heterotrophs evolve
65
archaea are mostly:
extremophiles
66
Thermophiles live in: Halophiles live in:
Thermophiles: live in hot springs & hydrothermal vents – 113 C Halophiles: highly saline environments
67
what are methanogens?
aerobes who use CO2 to oxidize H2 and give off methane
68
Prokaryotes play crucial roles in the :
biosphere
69
Lineages within Eukarya that are dominated by:
multicellular organisms (plants, fungi, and animals)
70
polytomy
a part of the tree where the relationship among the species is uncertain
71
why did the protista kingdom get abolished?
because it includes members that are more closely related to plants, fungi, or animals than to other protists
72
why did the kingdom monera get abolished?
because it would have members in two different domains.
73
4 types of spherical prokaryotes?
Cocci (singular, **coccus**) are spherical prokaryotes. pairs (**diplococci**), chains of many cells (**streptococci**) clusters resembling bunches of grapes (**staphylococci**)
74
2 types of rod-shaped prokaryotes
singular, bacillus rods are arranged in chains (streptobacilli)
75
In a ____ environment, most prokaryotes lose water and shrink away from their wall (____)
In a hypertonic environment, most prokaryotes lose water and shrink away from their wall (plasmolyze)
76
why is salt used to preserve food
because it causes prokaryotes to lose water, preventing them from rapidly multiplying.
77
what is eukaryote cell wall made of?
chitin or cellulose
78
bacterial cell wall is made of:
peptidoglycan
79
where is the genetic material located inside prokaryotes
their chromosome is located in the nucleoid, a region of cytoplasm that is not enclosed by a membrane
80
# reproduction why are prokaryotes so successful?
potential to reproduce quickly in a favourable environment