Limulus Lysate Test

Question 1

What is the purpose of the Limulus Lysate Test?

Answer 1

• Used for the diagnosis of meningitis caused by Gram-negative bacteria
• By detecting the reaction between Limulus amebocyte and the bacterial endotoxin of Gram-negative bacteria.

Question 2

What is the source of the reagent used in the Limulus Lysate Test?

Answer 2

blood cells of the Horseshoe Crab (Limulus polyphemus).

Question 3

What does a positive result in the Limulus Lysate Test indicate?

Answer 3

coagulation within 1 hour of incubation at 37°C.