Linked In OECD Guide

Question 1

OECD guide 202 Acute Daphnia Immobilization
Species:
#animals
Times
Test doses and design

Answer 1

Species: daphnia (magna)
#: >20
Times: 24hr and 48hr
Test doses/design: >=5 for EC50 immobilization or limit test dose 100mg/ml or max solubility

Question 2

OECD 203 Fish
Species
#animals
Times
Test dose and design

Answer 2

1. Danio rerio (zebra fish), fathead minnow, carp, guppy others
2. > =7 per dose
3. <24hr, 24hr, 96hr
4. > =5 for LC50 or limit test dose 100mg/ml or max solubility

Question 3

OECD 207 earthworm

Species
#animals
Times
Test dose and design

Answer 3

1. Eisenia foetida
2. > =10 per dose
3. 48hr, 72 optional (filter paper) 7 and 14days soil
4. > =5 for LC50 with optional prelim range finding with fewer worms(4) per dose

Question 4

OECD 408 repeat 90 day oral in rodents

Species
#animals
Times
Test dose and design

Answer 4

1. Rats, also mice
2. 20 animals per group (10m10f)
3. Daily x90 days
4. > =3 or limit dose 1000mg/kg

Question 5

OECD 413 90 day inhalation

Answer 5

1. Rat, also mice
2. > =20 per group(10m/10f)
3. Daily >=5x per week for 90 days
4. > =3 or limit at (5mg/L aerosol, 20mg/L vapor, 20,000ppm for gas) range finding with fewer animals per dose and >=1 dose

Question 6

OECD 414 prenatal developmental
Species
#animals
Times
Test dose and design
Stains

Answer 6

1. Rat and rabbit
2. > =20 animals with implantation
3. Daily from implantation to cesarean
4. > =3 or limit at 1000mg/kg
5. Ammonium sulfide, salewski

Question 7

OECD 420 acute oral
Species
#animals
Times
Test dose and design

Answer 7

1. Rats
2. 1 per dose sighting, 5 per main dose, F is normal
3. 3-4days between dose. Obs at 30min, <4hr, daily for 14days
4. Sighting study. Main test stepwise 5, 50, 300 and 2000, 5000 only if required

Question 8

OECD 432 in vitro 3t3 photo toxicity

Species
#animals
Times
Test dose and design
Stains

Answer 8

1. Mouse derived cell line
2. 2 96 well plates per chemical
3. 60 min dark exposure, 50 min UV exposure, 18-24hr incubation
4. 8 concentrations per chemical in light and dark
5. Neutral red

Question 10

OECD 437 bovine cornea opacity

Species
#animals
Times
Test dose and design

Answer 10

1. Bovine cornea
2. > =3 eyes per group
3. 10 min liquids, 4hr solids, 2hr rinse/remove liquids
4. Single dose of pure liquid or diluted solid with concurrent positive and negative controls

Question 11

OECD 438 isolated chicken eye
Species
#animals
Times
Test dose and design
Stains

Answer 11

1. Chicken eye
2. > =3 eyes per group
3. 10s exposure, 10s rinse, evaluate before 30s, 75, 120, 180 and 240 min
4. Undiluted liquids and powdered solids with positive and negative controls
5. Fluorescein