long question (4)

Question 1

you are working in a lab investigating fibrosis. you have samples from normal and fibrotic tissue. describe how you would use these samples to identify genes that might be relevant to fibrosis. your lab has a fibrosis cell line. describe experiments that would demonstrate the genes you identified are functionally relevant

Question 2

how would you use these samples to identify gene that may be relevant to fibrosis?

Answer 2

• check protein expression (western blotting)
• ELISA
• fluorescence
• compare normal and fibrotic tissue (gene expression)

Question 3

describe experiments that would demonstrate genes to identify are functionally relevant

Answer 3

• siRNA
• CRISPR