Media chart

Question 1

Type: Enriched
Differential: Hemolysis patterns

Answer 1

BAP (Sheep Blood Agar)

Question 2

Type: Selective for Gram-positive
Differential: Hemolysis patterns

Answer 2

CNA (Colistin Nalidixic Acid)

Question 3

Type: Selective for Gram-negative
Differential: Lactose fermentation
Results: LF = pink, NLF = clear (enteric pathogens)

Answer 3

MAC (MacConkey Agar)

Question 4

Differential: Sorbitol fermentation
Results: E. coli O157:H7 = clear, Serratia = red

Answer 4

MAC with Sorbitol

Question 5

Type: Selective for Gram-negative rods
Differential: Lactose fermentation
Result: E. coli = shiny metallic green

Answer 5

EMB (Eosin Methylene Blue)

Question 6

Type: Enriched for fastidious organisms
Contents: X & V factors
Organisms: Neisseria, Haemophilus, Moraxella

Answer 6

CHOC (Chocolate Agar)

Question 7

Type: Selective, chocolate-based
Contents: Nystatin (yeast), Vancomycin (GP), Colistin (GN), Trimethoprim (Proteus)
Organism: Neisseria

Answer 7

MTM (Modified Thayer Martin / Martin Lewis)

Question 8

Type: Selective & Differential for Salmonella & Shigella
Results: Shigella = green, Salmonella = black, Nonpathogens = yellow (LF)

Answer 8

HE (Hektoen Enteric Agar)

Question 9

Type: Selective & Differential for Salmonella & Shigella
Results: Shigella = red, Salmonella = black, Nonpathogens = yellow

Answer 9

XLD (Xylose Lysine Deoxycholate Agar)

Question 10

Type: Selective & Differential
Results: Vibrio cholerae = yellow, Other coliforms = green

Answer 10

TCBS (Thiosulfate-Citrate-Bile-Sucrose)

Question 11

Type: Selective for Campylobacter
Conditions: Incubate at 42°C
Contents: Brucella agar base + antibiotics + 10% sheep RBCs

Answer 11

CAMPY BAP

Question 12

Type: Selective for Yersinia
Results: Pink “bullseye” colonies
Condition: Incubate at 42°C

Answer 12

CIN (Cefsulodin-Irgasan-Novobiocin)

Question 13

Type: Fungal media

Answer 13

SAB (Sabouraud Dextrose Agar)

Question 14

Type: Selective & Differential
Results: Black colonies for Bacteroides fragilis, Group D Strep, Enterococcus

Answer 14

BE (Bile Esculin)

Question 15

Type: Selective & Transport medium
Organism: Bordetella pertussis

Answer 15

Reagan-Lowe

Question 16

Type: Selective
Organism: Legionella

Answer 16

BYE (Buffered Yeast Extract)

Question 17

Type: Selective
Organism: Francisella tularensis

Answer 17

Cysteine Glucose Agar

Question 18

Type: Enrichment
Enhances: Strep agalactiae (Group B Strep)

Answer 18

LIM Broth (Todd Hewitt

Question 19

Type: Enriched media
Organism: Mycobacterium tuberculosis (AFB)

Answer 19

Lowenstein-Jensen & Middlebrook (7H10, 7H11)

Question 20

Type: Nutrient broth/agar
Use: Aerobes & anaerobes (general growth)

Answer 20

THIO (Thioglycolate Broth)

Question 21

Selective for anaerobes

Answer 21

CDC-ANA (ANA-BAP)

Question 22

Type: For obligate & facultative anaerobes

Answer 22

BBA (Brucella Blood Agar)

Question 23

Type: Selective for bile-tolerant Bacteroides
Differential: B. fragilis = black colonies

Answer 23

BBE (Bacteroides Bile Esculin)

Question 24

Type: Anaerobic medium
Supports: Bacteroides & Prevotella
Inhibits: Other growth

Answer 24

LKV (Laked Kanamycin-Vancomycin Agar)

Question 25

Type: Supports anaerobes Inhibits: Enteric Gram-negatives

Answer 25

PEA (Phenylethyl Alcohol Agar

Question 26

Type: Nutrient medium Supports: All types of bacteria

Answer 26

Chopped Meat Broth

Question 27

Type: Selective & Differential Use: Stool enteric pathogens

Answer 27

GN & SF Broth

Question 28

Type: Selective for Staphylococcus Differential: Mannitol fermentation Results: S. aureus = yellow, Other staph = pink

Answer 28

Mannitol Salt Agar (MSA)