Methods

Question 2

Chemical method that includes alkaline copper reduction method? a. Condensation method b. Enzymatic method c. Oxidation reduction method d. Spectrophotometric method

Answer 2

c

Question 3

Sugar specificity of alkaline copper reduction method? a. Glucose only b. All carbohydrates c. All reducing sugars except sucrose d. Sucrose only

Answer 3

c

Question 4

Role of heat in alkaline copper reduction method? a. Inhibitor b. Stabilizer c. Catalyst d. Indicator

Answer 4

c

Question 5

Principle of alkaline copper reduction method? a. Oxidation of glucose to gluconic acid b. Reduction of cupric ions to cuprous ions in hot alkaline solution by glucose c. Condensation of glucose with aromatic amines d. Reduction of ferricyanide to ferrocyanide

Answer 5

b

Question 6

End product of alkaline copper reduction method? a. Cupric ions b. Cuprous ions c. Ferricyanide d. Ferrocyanide

Answer 6

b

Question 7

End color of Folin Wu method? a. Yellow b. Red c. Blue d. Colorless

Answer 7

c

Question 8

End color of Nelson Somogyi method? a. Blue b. Yellow c. Green d. Orange

Answer 8

a

Question 9

End color of Neocuproine method? a. Blue b. Yellow c. Red d. Colorless

Answer 9

b

Question 10

Benedicts method is a modification of which method? a. Nelson Somogyi b. Neocuproine c. Folin Wu d. Hagedorn Jensen

Answer 10

c

Question 11

Use of Benedicts method? a. Detection of glucose only b. Detection and quantification of reducing substances in blood and urine c. Measurement of ferricyanide d. Condensation reaction of glucose

Answer 11

b

Question 12

Stabilizing agent in Benedicts method? a. Oxalate b. Citrate or Tartrate c. Neocuproine d. Ferricyanide

Answer 12

b

Question 13

Effect if precipitate forms in Benedicts method? a. Increased color b. No color produced c. Yellow color forms d. Blue color intensifies

Answer 13

b

Question 14

Method classified as alkaline ferric reduction method? a. Folin Wu b. Benedicts c. Hagedorn Jensen d. Neocuproine

Answer 14

c

Question 15

Reaction involved in Hagedorn Jensen method? a. Reduction of cupric to cuprous ions b. Oxidation of glucose c. Reduction of yellow ferricyanide to colorless ferrocyanide d. Condensation with ortho toluidine

Answer 15

c

Question 16

Chemical method that includes ortho toluidine Dubowski method? a. Oxidation reduction method b. Enzymatic method c. Condensation method d. Ferric reduction method

Answer 16

c

Question 17

Method that is specific for glucose? a. Chemical method b. Enzymatic method c. Condensation method d. Ferric reduction method

Answer 17

b

Question 18

General rule regarding enzymatic method compared to chemical method? a. Less specific and less sensitive b. Same specificity but less sensitive c. Always superior in sensitivity and specificity d. Inferior in glucose measurement

Answer 18

c

Question 19

Enzymatic method that is second most specific? a. Hexokinase method b. Glucose oxidase method c. Benedicts method d. Ortho toluidine method

Answer 19

b

Question 20

Glucose form measured by glucose oxidase method? a. Alpha D glucose b. Beta D glucose c. L glucose d. Galactose

Answer 20

b

Question 21

Glucose oxidase method that produces a color reaction? a. Polarographic glucose oxidase b. Hexokinase method c. Saider Gernstenfield method d. Hagedorn Jensen method

Answer 21

c

Question 22

Glucose oxidase method that measures rate of oxygen consumption? a. Colorimetric glucose oxidase b. Polarographic glucose oxidase c. Hexokinase method d. Neocuproine method

Answer 22

b

Question 23

Interpretation of oxygen consumption in polarographic glucose oxidase method? a. Inversely proportional to glucose concentration b. Equal to glucose concentration c. Independent of glucose concentration d. Only qualitative

Answer 23

b

Question 24

Substance that causes false decreased glucose in glucose oxidase method? a. Bleach b. Ascorbate c. Glucose d. Sodium fluoride

Answer 24

b

Question 25

Substance that causes false increased glucose in glucose oxidase method? a. Bilirubin b. Uric acid c. Bleach d. Creatinine

Answer 25

c

Question 26

Reference method for glucose determination? a. Glucose oxidase method b. Hexokinase method c. Benedicts method d. Folin Wu method

Answer 26

b

Question 27

Most specific enzymatic method for glucose? a. Glucose oxidase method b. Hexokinase method c. Polarographic method d. Colorimetric method

Answer 27

b

Question 28

Sample used for hexokinase method? a. Serum only b. Whole blood only c. Plasma using heparin EDTA fluoride oxalate or citrate d. Urine only

Answer 28

c

Question 29

Additional samples applicable for hexokinase method? a. Serum and plasma only b. Urine CSF and serous fluids c. Whole blood only d. Saliva and sweat

Answer 29

b

Question 30

Substance that does not affect hexokinase method? a. Ascorbic acid b. Uric acid c. Both ascorbic acid and uric acid d. Bleach

Answer 30

c

Question 31

Cause of false decreased plasma glucose in hexokinase method? a. Bleach b. Hemolysis and ictericia c. Ascorbic acid d. Creatinine

Answer 31

b

Question 32

Most commonly used enzymatic method for lactate

Answer 32

lactate oxidase

Question 33

usually satisfactory additives for lactate

Answer 33

iodoacetate and fluoride

Question 34

CDC Reference Method for TAG

Answer 34

Colorimetric Method (Van Handel & Zilversmith)

Question 35

Colorimetric Method (Van Handel & Zilversmith) is what color?

Answer 35

Blue

Question 36

Fluorometric Method for detecting TAG

Answer 36

Hantzsch Condensation

Question 37

Hantzsch Condensation forms what color?

Answer 37

Blue

Question 38

TAG's latest reference method

Answer 38

GCMS

Question 39

Enzymatic method for TAG

Answer 39

Glycerol Kinase Method

Question 40

hydrolysis of TAG to free fatty acids and glycerol

Answer 40

then phosphorylation of glycerol to glycerophosphate

Question 41

Chemical methods for 3-hydroxy-56-cholestene

Answer 41

lieberman buchardth reaction and salkowski reaction

Question 42

Liebarman burchardt reaction forms what color?

Answer 42

green

Question 43

Salkowski reaction forms what color?

Answer 43

red

Question 44

CDC reference method for cholesterol

Answer 44

abell

Question 45

the CDC reference method for cholesterol uses what extraction after hydrolysis with alcoholic KOH followed by Liebermann burchrdt color rgt

Answer 45

hexane

Question 46

latest reference method that specifically measures cholesterol

Answer 46

GC-MS