Microbial Genetics Flashcards

(21 cards)

1
Q

Nucleotide base

A

Nucleotide Monomer

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2
Q

How does DNA turn into proteins in a cell?

A

Transcription - DNA is copied into single stranded RNA
Translation - RNA is decoded to make proteins using ribosomes

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3
Q

Gene

A

A unit of the cells DNA that encodes a specific protein

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4
Q

What happens if a gene is expressed?

A

It is transcribed into RNA and then translated into a protein

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5
Q

If a gene is not expressed ______.

A

No RNA or protein is made.

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6
Q

Types of chromosomal genes in the bacterial genome

A

Constitutively expressed - Almost always expressed
Inducible - Turned on when needed
Repressible - Turned off when not needed

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7
Q

Types of plasmid genes in the bacterial genome

A

Not needed for survival
Conjugative - genes for conjugation and sex pilus
Antibiotic resistance - genes for antibiotic resistance
others - nitrogen fixation, oil degredation, defense,

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8
Q

Base pair substitution

A

Changes 1 base, doesn’t change the mRNA

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9
Q

Frame shift mutation

A

Inserts or deletes multiple nucleotides, changes downstream protein code

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10
Q

Synonymous mutation

A

No change in protein

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11
Q

Missense Mutation

A

Changed amino acids

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12
Q

Nonsense Mutation

A

Protein translation stopped

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13
Q

Transformation (horizontal gene transfer)

A

Naked DNA is taken up by a competent bacteria (bacteria that can take up naked DNA)

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14
Q

Conjugation (horizontal gene transfer)

A

An F+ bacteria touches another bacteria with it’s sex pilus and transfers genetic info to it

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15
Q

Transduction (horizontal gene transfer)

A

Bacteriophage accidentally takes some bacterial DNA and passes it on to other bacteria

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16
Q

DNA

A

self-replicating material that is the carrier of genetic information

17
Q

Genome

A

the complete set of genetic material, DNA or RNA, in an organism, containing all of its genes and other information

18
Q

Gene

A

a fundamental unit of heredity, a segment of DNA that contains the instructions for building proteins or functional RNA molecules

19
Q

what time of macro molecule is DNA

20
Q

lac operon

A

set of genes in E. coli and other bacteria that enables them to transport and metabolize lactose for energy when glucose is unavailable

21
Q

why do bacteria need horizontal and vertical genetics

A

vertical transfer (VGT) to pass on traits to their offspring, ensuring species identity and maintaining established genetic information, and for horizontal transfer (HGT) to rapidly acquire new traits from other bacteria