The image that appears on the microscope when light projected towards an object, bounces off the object and passes through a lens to form a magnified image.
Virtual Image
The ability to distinguish objects that are close together. Determines the size of the smallest object that can be discerned under operating conditions
The amount of detail that can be ascertained by the observer and also refers to the minimum distance between points that the observer can distinguish
Resolving Power (Resolution)
The distance between the slide and the bottom of the objective lens
Working Distance
The degree to which light bends as it passes from one medium (glass, oil, air) to another during formation and projection of an image through a microscopic lens
Refraction
The eye-piece of the microscope
Ocular lens
Because the microscope is _______, the object in view under a low power objective should still be in view under a higher power lens.
Parfocal.
Do not have to adjust the focus knobs.
As you increase magnification power, the _______ will decrease.
Diameter of the field of view
(Inverse relationship)
A special type of synthetic oil
Immersion oil
Placed on the slide in between the specimen and the oil immersion objective (100x)
What is the purpose of immersion oil?
To prevent light rays from bending as they pass through the slide to the objective lens.
Immersion oil has the same refractive index as ______.
glass
When using the oil immersion objective, it is imperative that the _______ not be used because the slide may crack and the objective may be damaged.
coarse adjustment
The working distance _____ as magnification _____ due to the higher numerical apertures associated with high power objectives
decreases; increases
Be careful not to place the high power objective in the oil. Always move from ___X to ___X to ___X. Avoiding the ___X objective.
10X; 4X; 100X;
Avoid the 40X
What is the total magnification under the oil immersion objective?
1000x
What is the scanning objective?
The low power objective (4X)
When putting away the microscope, how should you position the objectives and stage?
Position the scanning objective in the viewing position and lower the stage fully away from the objectives array in the nosepiece.
Light intensity should be set at ____.
3.5
