1
Q
Motility indole ornithine (Gram -)
A
Semi-solid )tempered medium
2
Q
Motility?
A
Turbidity away from stab line
3
Q
Does bacteria have tryptophanase?
A
If present, indole pyruvic acid formed from broken down tryptophan
4
Q
Indole deamination
A
When pH moved to acidic, indole pyruvic acid separated into indole + pyruvic acid
5
Q
Kovac’s reagent
A
Reacts with indole, forms red ring at top of medium
6
Q
Ornithine
A
Does bacteria possess ornithine decarboxylase?
7
Q
Decarboxylation loses CO2, putrescine
A
Putrescine produced = raised pH = turns purple when pH > 7.6
8
Q
Bromocresol purple
A
Glucose turns media yellow, then back to purple. If stays yellow, glucose metabolized and putrescine not produced.
9
Q
Ingredients
A
Tryptophan
Kovac’s reagent
Ornithine
Bromocresol purple
Microbio
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