Molecular Techniques

Question 1

Southern Blot

Answer 1

DNA
Detect large changes in DNA
Point mutations associated with restriction sites

Question 2

Northern blot

Answer 2

RNA

Measures mRNA amount and size

Question 3

Western blot

Answer 3

Proteins

Question 4

ASO (probes)

Answer 4

DNA
Small changes in DNA

Used with southern blots

Question 5

Microarray

Answer 5

CDNA or genomic DNA, proteins

Measures expression

Question 6

ELISA

Answer 6

Proteins
Detects antigens or antibodies

Low sensitivity (false positives) prefer ELISA

Question 7

PCR amplification

Answer 7

DNA replication

SDS page gell: smaller pieces go farther

Question 8

RFLP: restriction fragment length polymorphism

Answer 8

Detects SNPs and DNA length