Outbreak

Question 1

equation for number of samples

Answer 1

(100/x) x 3

Question 2

benefits of using reverse pipetting

Answer 2

-accuracy
-minimise splashing/spread

Question 3

Name and briefly describe typing methods that you might use in an outbreak to identify a pathogen

Answer 3

-PCR - section , can’t see alive or dead
-phage typing - section , strains
-pulsed field gel electrophoresis - whole genome , different strains
-serotyping - find serotype , agglutination
-IMViC tests - to differentiate between coliforms
-gene sequencing - PCR but of gene or whole genome