1
Q
methods to determine primary structure?
A
chemical- edman deg
physical- MS
biological- DN sequencing, next gen seq
2
Q
how does chemical peptide cleavage occur?
A
cyanogen bromide
3
Q
how does enzymatic peptide cleavage occur?
A
trypsin or chymotrypsin
4
Q
edman method of protein sequencing
A
disulfide bonds reduced - GluHCL
sanger reagent
5
Q
advantages of MS over Edman
A
more sensitive, faster
doesnt require pure proteins
identifies blocked/mod proteins
