1
Q
types of energy transitions in molecules
A
rotational
vibrational
electronic
2
Q
what type of excitation is UV-Vis?
A
electronic
3
Q
what is a chromophore?
A
a molecular structure that interacts with photon
4
Q
what occurs if titratable group becomes charged?
A
lambda max and e increase
bathochromic hyperchromic shifts
5
Q
what are the chromophores in proteins?
A
peptide bonds
aa side chains
prosthetic groups
6
Q
what biomolecules do not have characteristic UV-VIS absorption?
A
mono/oligosaccharides
lipids
many metabolites
7
Q
why do polynucleotides have reduced absorbance?
A
stacking interactions between bases - hypochromicity
