MAJOR INTRACELLULAR ANION
Component of phospholipids, nucleic acids, creatine phosphate and ATP
PHOSPHATE
Phosphate in diet is MAXIMALLY absorbed in the jejunum
Small Intestine
Percentage of Phosphate in bones
85%
Percentage of Phosphate in extracellular environment
15%
found in many parts of tissues/cells
Omnipresent
T/F. Growth Hormone increases renal excretion of phosphate
False, decreases renal excretion
T/F. The level of phosphate is INVERSELY RELATED TO CALCIUM
True
T/F. In the kidneys through the action of the PTH, calcium is absorbed while phosphate is excreted.
True
FORMS OF PHOSPHATE IN CELLS
Organic
Inorganic
Inside the cell; the principal anion in the cell
Organic
Outside the cell; blood buffer present in the plasma or serum
The one measured in tests
Inorganic
CAUSE OF HYPOPHOSPHATEMIA
Hyperparathyroidism (↑ renal excretion)
Vitamin D Deficiency or antacid use (↓intestinal absorption)
CAUSE OF HYPERPHOSPHATEMIA
Hypoparathyroidism
Lymphoblastic leukemia, intensive exercise, neoplastic disorders
Hypervitaminosis
What are the tube and specimen needed for Specimen Collection of Phosphate?
Serum, Plasma (lithium heparin), 24-hour urine
T/F. Hemolysis cause False ↑
True
Absorbance of (340 nm)
Ammonium phosphomolybdate complex
Fiske-Subbarow Method
o Reducing agent:
Pictol (Amino Naphthol Sulfonic acid)
Fiske-Subbarow Method
o Other reducing agents:
Elon or Methyl Amino Phenol
Ascorbic acid
Senidine or N-Phenyl-P PhenyleneDiamineHyrochloride