PLATE COUNT METHOD

Question 1

T/F:
You count colonies instead of bacterial cells?

Answer 1

TRUE

Question 2

What is the Standard Plate Count (SPC)

Answer 2

colonies that are seen on the plates after incubation represent living organisms

Question 3

What the Colony-Forming Units (CFU)?

Answer 3

when the number of viable bacteria in a sample is expressed as the “number of colonies”

Question 4

What was the mechanism used to calculate ever colony on the plate?

Answer 4

Quebec Colony Counter

Question 5

How to calculate the number of organisms per ml of culture?

Answer 5

multiply the number of colonies counted on the plate by the dilution factor (i.e, 10^-6)

CFU = number of colonies / dilution factor