in the mitosis practical why is
- Hydrochloric acid
- A stain
- Mounted needle
- root tip
needed ?
- hydrochloric acid - softens and loosens the root tip
- stain - it dyes the chromosomes to make them visible
- mounted needle - To lower the coverslip preventing air bubbles
- root tip - where the region of growth is so that is where mitosis occurs
Why do we need to squish the root tip in the mitosis practical
So you can get a thin layer of cells so light can pass through
What are some of the hazards in the mitosis practical
-scapple - cutting yourself while using it - cut on top of a white tile to prevent slipping
- hydrochloric acid - irritant - wear safety goggles
How do you work out the mitotic index
what do you do to make sure your cell count is accurate
number of cells going trough mitosis / total number of cells
x100
examine a large number of fields of view or many cells
how do you convert mm into um and vice-versa
mm into um = x1000
um into mm = /1000
What is an equation for volume of a new solution by a dilution series
c1xv1= c2xv2
conc of stock solution x volume of stock solution = conc of new solution x volume of new solution
