Processing Samples and Lab. Diagnosis for Mycobacterium

Question 1

This is done in order to free and visualize the bacilli stuck inside the mucoid layers of the sputum? a. Neutralize b. Liquefy c. Decontaminate d. Culture

Answer 1

b

Question 2

Gold standard mucolytic agent for sputum processing? a. Sodium Hydroxide b. Zephiran c. N-Acetyl L-cysteine d. 6% oxalic acid

Answer 2

c

Question 3

An alternative to N-Acetyl L-cysteine? a. Dithiothreitol b. Tween 80 c. Cyanogen Bromide d. Tellurite

Answer 3

a

Question 4

Gold standard decontaminating agent? a. 6% oxalic acid b. Sodium Hydroxide c. Zephiran d. Buffer

Answer 4

b

Question 5

Used for highly contaminated samples if there is the presence of other Gram-negative bacilli Pseudomonas and Proteus? a. Sodium Hydroxide b. Zephiran c. Buffer d. 6% oxalic acid

Answer 5

d

Question 6

To qualify whether to accept or reject the sample? a. Acid Fast Stain b. Culture c. Gram Stain d. Niacin test

Answer 6

c

Question 7

For the sample to be accepted WBCs or macrophages must be seen in? a. Culture b. Gram Stain c. Acid Fast Stain d. Arylsulfatase test

Answer 7

b

Question 8

Preferred staining method? a. Gram Stain b. Culture c. Acid Fast Stain d. Catalase test

Answer 8

c

Question 9

1-2 per 300 fields in Acid Fast Stain? a. +1 b. +2 c. Doubtful request another specimen d. +3

Answer 9

c

Question 10

1-9 per 10 fields in Acid Fast Stain? a. +2 b. +3 c. +4 d. +1

Answer 10

a

Question 11

Greater than 9 AFB per field? a. +3 b. +1 c. 0 d. +4

Answer 11

d

Question 12

The following are AGAR BASE MEDIA for susceptibility assays EXCEPT? a. Dubois’s Oleic Acid Albumin medium b. Mitchison’s medium c. Lowenstein-Jensen medium d. Middlebrook 7H10-7H11

Answer 12

c

Question 13

Used for AST? a. Petragnani medium b. Middlebrook 7H10-7H11 c. Dorset Egg medium d. Bactec 12B

Answer 13

b

Question 14

Most widely used among egg-based media? a. Petragnani medium b. American Thoracic Society medium c. Lowenstein-Jensen medium d. Dorset Egg medium

Answer 14

c

Question 15

Contains the highest malachite green concentration for highly contaminated samples? a. Petragnani medium b. Lowenstein-Jensen medium c. Dorset Egg medium d. Middlebrook 7H

Answer 15

a

Question 16

The following are LIQUID MEDIA EXCEPT? a. Bactec 12B b. Septi-Chek AFB c. Middlebrook 7H d. Dorset Egg medium

Answer 16

d

Question 17

Niacin plus Niacin Ribonucleotide plus Aniline Dye plus Cyanogen Bromide produces? a. Pink color b. Yellow-colored complex c. Red color d. Black metallic tellurium

Answer 17

b

Question 18

Positive control for Niacin test? a. Mycobacterium bovis b. Mycobacterium avium c. Mycobacterium kansasii d. Mycobacterium tuberculosis

Answer 18

d

Question 19

Negative control for Niacin test? a. Mycobacterium bovis b. Mycobacterium tuberculosis c. Mycobacterium avium d. Mycobacterium kansasii

Answer 19

a

Question 20

Reagent used in Catalase test? a. HCl b. 30% H2O2 c. Sulfanilamide d. Alpha naphthyl amine

Answer 20

b

Question 21

Heat stable catalase test temperature? a. 56ºC b. 60ºC c. 68ºC d. 70ºC

Answer 21

c

Question 22

Negative control for Catalase test due to its heat sensitive catalase activity? a. Mycobacterium kansasii b. Mycobacterium avium c. Mycobacterium tuberculosis d. Mycobacterium fortuitum

Answer 22

c

Question 23

Nitroreductase is detected by all of the following EXCEPT? a. HCl b. Sulfanilamide c. Alpha naphthyl amine d. Cyanogen Bromide

Answer 23

d

Question 24

Positive result of Nitrate Reduction test? a. Yellow-colored complex b. Red c. Pink color d. Black metallic tellurium

Answer 24

c

Question 25

Detects rapid growers? a. Arylsulfatase test b. Niacin test c. TCH Susceptibility test d. Tellurite test

Answer 25

a

Question 26

Negative results automatically indicate slow growers in? a. Tween 80 HOH test b. Arylsulfatase test c. Catalase test d. Nitrate Reduction test

Answer 26

b

Question 27

Tripotasium Phenolphthalein to Free Phenolphthalein is the principle of? a. Tellurite test b. Niacin test c. Arylsulfatase test d. TCH Susceptibility test

Answer 27

c

Question 28

Principle of Tween 80 HOH test? a. Tellurite to Black Metallic Tellurium b. Hydrolysis of Tween 80 c. Nitroreductase d. Thiophene-2-carboxylic acid hydrazide

Answer 28

b

Question 29

Positive control for Tween 80 HOH test? a. Mycobacterium kansasii b. Mycobacterium avium c. Mycobacterium tuberculosis d. Mycobacterium bovis

Answer 29

a

Question 30

Tellurite produces? a. Yellow-colored complex b. Pink color c. Black metallic tellurium d. Red

Answer 30

c

Question 31

TCH stands for? a. Tripotasium Cyanogen Hydrazide b. Thiophene-2-carboxylic acid hydrazide c. Tween Catalase Hydrazide d. Thiocarboxyl hydrazide

Answer 31

b

Question 32

Susceptible in TCH Susceptibility test? a. Mycobacterium tuberculosis b. Mycobacterium avium c. Mycobacterium kansasii d. Mycobacterium bovis

Answer 32

d

Question 33

Resistant in TCH Susceptibility test? a. Mycobacterium tuberculosis b. Mycobacterium bovis c. Mycobacterium avium d. Mycobacterium fortuitum

Answer 33

a