Readings

Question 1

whats a big diff in 4th gen vs 3rd gen

Answer 1

instead of having all the reads attributing back to a sample, were attributing them back to a cell type (like DNA in semen vs DNA in blood)

Question 2

which gen is sanger

Answer 2

1

Question 3

what did figure 2 of lecture 1 look at

Answer 3

Missing heritability in psychiatric disease risk and in the metabolism of antidepressants and antipsychotic

Question 4

what kind of studies were used to determine missing heredibility

Answer 4

twin studies

Question 5

what are rare variations defined as

Answer 5

frequencies of the minor allele of less than 1%

Question 6

what were the quality results in that paper on HTS in ponds

Answer 6

high percentage of sufficient quality

Question 7

which sample methods were best/worst in the paper on HTS in ponds

Answer 7

• swabs were worse (particularly gill)
• skin swabs meh
• illumina was best

Question 8

fish euthanized with MS-222 trended towards _____ (higher/lower) alpha diversity compared with those euthanized by cranial concussion and subsequent pithing

Answer 8

higher

Question 9

what is the central dogma of molecular biology

Answer 9

• DNA
• mRNA
• protein
• metabolite
• microbe

Question 10

what info can you get from genomics/ epigenomics

Answer 10

• cause of death
• age of death
• sex, ancestry, phenotype

Question 11

what info can you get from transcriptomics

Answer 11

PMI

Question 12

what info can you get from proteomics

Answer 12

• cause of death
• age of death
• sex, ancestry,, phenotype
• PMI

Question 13

what info can you get from metabolomics, lipidomics

Answer 13

• cause of death
• PMI

Question 14

what info can you get from microbiomics

Answer 14

• cause of death
• age at death
• PMI

Question 15

when gen is illumina

Answer 15

2

Question 16

which gen is ion torrent

Answer 16

2

Question 17

which gen is pacbio

Answer 17

3

Question 18

which gen is nanopore

Answer 18

3