Matter interacts with light by absorbing and transmitting different wavelengths.
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Spectrophotometry quantitatively describes either the transmittance or absorbance of light by a substance.
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The optimal light wavelength to use for analysis is denoted λmax and is the wavelength at which maximal light absorbance occurs.
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Beer-Lambert Law states that a substance’s absorbance value is directly proportional to its concentration.
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If absorbance can be measured, it can be used to determine the concentration of an unknown solution.
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If a solution is not coloured it is often possible to engineer a situation in which is undergoes a reaction or is conjugated to a coloured compound.
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