starting

Question 1

how to isolate a protein

Answer 1

take gene of interest insert via a plasmid -> transfect the cell -> host exspansion in bioreactor -> protein purification

Question 2

name two digestive proteins

Answer 2

trypsin
HIV protease

Question 3

What are three methods to identify protein structure

Answer 3

protein crystalography
cryo-electron microscopy
NMR spectroscopy

Question 4

name 3 Metabolisum linked proteins + function

Answer 4

alcohol dehydrogenase - metabolize ethaol
hexokinase - binds phosphate groups to glucose