Sterilizing and Hygiene

Question 1

How can you kill bacterial spores?

Answer 1

Spores are highly resistant to heat and chemicals

Must autoclave to kill spores by steaming at 121 deg C for 15 minutes

Question 2

How can you kill exotoxins?

Answer 2

Destroyed rapidly at 60C (except staph enterotoxin and E coli heat-stable toxin)

Question 3

How can you kill endotoxins?

Answer 3

Stable at 100C for 1 hour