Topic 7: Microbial Systematics

Question 1

3 major domains

Answer 1

• bacteria
• archaea
• eukarya

Question 2

Bacteria (domain)

Answer 2

• Peptidoglycan cell walls

- Most prokaryotes

Question 3

Archaea (domain)

Answer 3

Prokaryotes that live in extreme environments but is also symbiotic with animals

Question 4

Eukarya (domain)

Answer 4

Animal, plants, fungi

• membrane bound nucleus
• more related to archaea than bacteria

Question 5

Archaea membrane phospholipids

Answer 5

Bipolar and form a monolayer

Question 6

Archaea cell wall

Answer 6

Made of pseudopeptidoglycan

Question 7

Serological tests

Answer 7

If two bacterium are evolutionarily close, antibodies by one bacteria will recognize and attach to the other
- If not, they do not react

Question 8

Slide agglutination test

Answer 8

• serological test
• Antiserum from one bacterial strain is placed onto a slide with unknown culture
• If they are from the same serotype, the cells will clump together

Question 9

Direct ELISA

Answer 9

Looking to see if a clinical sample has a specific microbe in it

Question 10

Indirect ELISA

Answer 10

Looking to see if a clinical sample has a specific antibody for a particular microbe

Question 11

Phage typing

Answer 11

Infecting the bacteria with a certain type of bacteriophage

• Only infects certain bacteria
• If a plaque is formed, the bacteria are from a certain species