Transformation

Question 1

Transformation

Answer 1

The ability of a bacteria to…take up DNA fragments from the environment and express the information encoded by the gene

Question 2

Resistance

Answer 2

Bacteria can pick uo or exchange genes for antibiotic resistance

Question 3

Molecular techniques/subcloning

Answer 3

-plasmids can be used to introduce foreign DNA into bacteria for subcloning purposes

Question 4

Plasmid

Answer 4

Self replicating, circular, dsDNA

Question 5

Transformation procedure

Answer 5

• choose a gene of interest and place it in a plasmid
• the plasmid can be transformed into E. coli MM294 cells
  
  • E. coli miseducating into a CaCl2 solution
  • heat shocked–>42’C for 90 seconds
  • the cells are plated on plates containing ampicillin
  • E. coli cells without plasmid= no growth
  • transformed E.coli with plasmid= growth
• transformed strain can now be isolated/cultured so the plasmid can replicate
• retrieve the plasmids from the cells and subsequently several copies of gene of interest