what is the principle behind field flow fractionation?
separation field vs forces of diffusion
distribution of the particles in the different layers of the parabolic flow is greater than separation
the crossflow separation field is perpendicular to what?
the channel
what are examples of two configurations of field flow fractionation?
asymmetric flow field flow fractionation (AF4)
hollow fiber flow field flow fractionation (HF5)
what is a fractogram?
diagram representing the dector signal vs time during the FF separation
what are advantages of field flow fractionation over other separation techniques?
- no stationary phase (no sample breakthrough effects of sample loss)
- upper limit of FF extends to 10^16 Da or wide dynamic particle size range is achievable
- works on aqueous and organic solvent carriers (versatile in terms of density, solubility ,etc…)
- mild operation conditions (good for fragile analytes)
- particles can be measured in dispersion without shape change
define omics
the analysis of interactions of biological information objects in various “omes”
what are main focuses of omics?
- mapping information objects (eg. genes, proteins, ligands)
- finding interaction relationships among objects
- engineering networks and objects to understand and manipulate regulatory mechanisms
- integrating omes and omic subfields
define foodomics
study of food domains in application of omic technologies to improve consumers well-being, health and confidence
define metabolomics
study of global changes in the entire metabolite set of certain cells, tissues, organs and organisms
applied in fields such as medical, environment, food science
describe the workflow of a typical metabolomics
- sample prep to extract the metabolome
- screening of the extract with advanced analytical tools
- data trt
- interpretation of results
what are examples of applications of foodomics?
- identification of prohibited substances
- characterization of GM foods
- discovery of new dietary markers
- food authenticity
